Flow Cytometers
Straight-forward setups for pollen vitality measurements
Reagents
Reagents for the Flow Cytometry analyses
Coming up
For future products keep an eye on this page
Products
Flow Cytometers
Configured for Pollen Vitality measurement
Making crosses usually is a straightforward process of taking pollen from one parent and placing them on another. But for many crops the results of these crossings can vary. There are multiple factors that influence the success rate of a crossing, but a major reason for low or no seed yield is low vitality of the pollen. The vitality of pollen can be influenced by many factors, including genetics, climate conditions, moment of collecting and time between collecting and pollination.
Pollen vitality can easily and accurately be measured using flow cytometry. Using flow cytometry over 10.000 pollen can be measured individually in just one minute. With this tool, a breeder can gain valuable information about the right moment to make a crossing and the optimal storage conditions of the pollen.
Download PDF
Flow Cytometers
Microspore and Pollen Vitality | Ploidy level | Aneuploidy
Thoughout the years we used flow cytometry in various projects for our Plant Breeder customers. Pollen Vitality determination is a frequently used application of flow cytometry, giving us the opportunity to add value as a Breeding Support Laboratory. Because of the degenerative nature of most pollen, we decided to sell flow cytometers configured specifically for pollen vitality analysis.
For Microspore viablity a similar configuration and setup can be used.
Other applications with our flow cytometers are the determination of ploidy level, and detection of aneuploids in batched samples.
Flow Cytometers
Microspose and Pollen Vitality | Ploidy level | Aneuploidy
Thoughout the years we used flow cytometry in various projects for our Plant Breeder customers. Pollen Vitality determination is a frequently used application of flow cytometry, giving us the opportunity to add value as a Breeding Support Laboratory. Because of the degenerative nature of most pollen, we decided to sell flow cytometers configured specifically for pollen vitality analysis.
For Microspore viablity a similar configuration and setup can be used.
Other applictions with our flow cytometers are the determination of ploidy level, and detection of aneuploids in batched samples.
Pollen Vitality
Flow Cytometers configured to measure pollen vitality
The ‘Quantum P’ flow cytometer in combination with the software ‘CyPAD’ makes measuring pollen vitality a simple and cost-effective process:
- Collect the pollen & mix them with the provided buffer,
- Add the provided reagent, and wait ~15 minutes,
- Insert the tube in the machine and directly see the results.
After the collection of the data, it can easily be exported to a spreadsheet to be freely customised. Optionally, the process can be partially automated using an auto-loader (more information will follow). For more information about our method, a demonstration, pricing or any other question please contact one of our experts via the buttons below.
Flow cytometer diagram of vital pollen
Pollen Vitality
A tool for in plant breeding and seed production
The ‘Quantum P’ flow cytometer in combination with the software ‘CyPAD’ makes measuring pollen vitality a simple and cost-effective process:
- Collect the pollen & mix them with the provided buffer,
- Add the provided reagent, and wait ~15 minutes,
- Insert the tube in the machine and directly see the results.
After the collection of the data, it can easily be exported to a spreadsheet to be freely customised. Optionally, the process can be partially automated using an auto-loader (more information will follow). For more information about our method, a demonstration, pricing or any other question please contact one of our experts via the buttons below.
Download PDF
Flow Cytometers...
for Pollen Vitality
Maximize your crossing success rate
Our fully configured flow cytometry setup gives plant breeders and seed producers acces to on-site measurements of pollen and microspore vitality.
With this powerful indicator come several possiblities, leading to an increased success rate of crossings:
- Optimise timing of hybridisation
- Determine optimal storage conditions
- Selection of preferred male lines
With 3 simple steps, and 20-30 samples / hour, our method, is easy to learn and implement in your crossing program.
Flow Cytometers...
for Pollen Vitality
Maximize your crossing success rate
Our fully configured flow cytometry setup gives plant breeders and seed producers acces to on-site measurements of pollen and microspore vitality.
With this powerful indicator come several possiblities, leading to an increased success rate of crossings:
- Optimise timing of hybridisation
- Determine optimal storage conditions
- Selection of preferred male lines
With 3 simple steps our method, is easy to learn and implement in your crossing program.
Pollen vitality analysis
in 3 Steps
- Mix collected pollen with provided buffer
- Add reagent, wait ~15 min
- Insert tube in the flow cytometer, real time results!
Download & Share our leaflet on Pollen Vitality Measurements:
Pollen Vitality
Flow Cytometers configured to measure pollen vitality
CyPAD dot-plot showing 𝘉𝘳𝘢𝘴𝘴𝘪𝘤𝘢 𝘯𝘢𝘱𝘶𝘴 pollen grains (green: vital, red: non-vital, grey: other particles) with a vitality score of 45%
Our fully configured flow cytometry setup gives plant breeders and seed producers acces to on-site measurements of pollen and microspore vitality.
With this powerful indicator come several possiblities, leading to an increased success rate of crossings:
- Optimise timing of hybridisation
- Determine optimal storage conditions
- Selection of preferred male lines
With 3 simple steps, and 20-30 samples / hour, our method, is easy to learn and implement in your crossing program.
3 steps:
- Mix collected pollen with provided buffer
- Add reagent, wait ~15 min
- Insert tube in the flow cytometer, real time results!
Download & Share Leaflet on Pollen Vitality Measurements:
Flow Cytometers
Configured for Pollen Vitality measurement
Making crosses usually is a straightforward process of taking pollen from one parent and placing them on another. But for many crops the results of these crossings can vary. There are multiple factors that influence the success rate of a crossing, but a major reason for low or no seed yield is low vitality of the pollen. The vitality of pollen can be influenced by many factors, including genetics, climate conditions, moment of collecting and time between collecting and pollination.
Pollen vitality can easily and accurately be measured using flow cytometry. Using flow cytometry over 10.000 pollen can be measured individually in just one minute. With this tool, a breeder can gain valuable information about the right moment to make a crossing and the optimal storage conditions of the pollen.
Flow Cytometers configured to measure pollen vitality
Flow Cytometers configured to measure pollen vitality
The ‘Quantum P’ flow cytometer in combination with the software ‘CyPAD’ makes measuring pollen vitality a simple and cost-effective process:
Collect the pollen & mix them with the provided buffer,
Add the provided reagent, and wait ~15 minutes,
Insert the tube in the machine and directly see the results.
After the collection of the data, it can easily be exported to a spreadsheet to be freely customised. Optionally, the process can be partially automated using an auto-loader (more information will follow). For more information about our method, a demonstration, pricing or any other question please contact one of our experts via the buttons below.
Reagents
Buffers and stainings for Flow cytometric analyses
For flow cytometric analyses we provide all the required reagentia & buffers:
Pollen Vitality analysis
– Pollen Buffer: 500ml in glass bottle (for 500 samples)
– FDA staining: 0,5ml in Eppendorftube (for 500 samples)
Coming up...
We are working hard to add new products to our website. Products that are already available through Iribov Innovations:
Flow cytometers configured for
– Ploidy determination
– Aneuploidy determination
– Absolute DNA content
Products that will be available in the future:
– Software for Tisscue Culture Laboratories
– Other Tissue culture related solutions
We are happy to help you on your next project
Contact our technical account manager for inquiries or a quotation
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